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Endress+Hauser inc flexcycler pcr system
Flexcycler Pcr System, supplied by Endress+Hauser inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flexcycler+pcr+system/flexcycler2+thermocycler/pmc11512275-150-7-10
Average 90 stars, based on 1 article reviews
flexcycler pcr system - by Bioz Stars, 2026-10
90/100 stars

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Reverse Transcription:

Article Title: Detection and Quantification of Soil-Borne Wheat Mosaic Virus, Soil-Borne Cereal Mosaic Virus and Japanese Soil-Borne Wheat Mosaic Virus by ELISA and One-Step SYBR Green Real-Time Quantitative RT-PCR
Article Snippet: First, 1× ProtoScript ® II reaction buffer, 10 μL of random primers (final concentration of 2 μM), 2 μL DTT, 20 U ProtoScript ® II Reverse Transcriptase (New England Biolabs) and 125 μmol of each dNTP (12.5 μM) were combined in a 20 μL reaction mixture. .. Then, reverse transcription was performed in a FlexCycler PCR System (Analytik Jena, Germany) with the following protocol: 25 °C for 5 min, reverse transcription at 42 °C for 1 h followed by a step of 20 min at 65 °C for inactivation of the enzyme. .. The obtained cDNA was then used as the template (2 μL) in a 20 μL polymerase chain reaction (PCR) mix containing 500 nmol of the respective primer set to detect SBWMV, SBCMV or JSBWMV RNA1 or RNA2 , 125 μmol of each dNTP, 4 μL of 5× buffer and 0,5 U One Taq polymerase (New England Biolabs).

Polymerase Chain Reaction:

Article Title: Detection and Quantification of Soil-Borne Wheat Mosaic Virus, Soil-Borne Cereal Mosaic Virus and Japanese Soil-Borne Wheat Mosaic Virus by ELISA and One-Step SYBR Green Real-Time Quantitative RT-PCR
Article Snippet: First, 1× ProtoScript ® II reaction buffer, 10 μL of random primers (final concentration of 2 μM), 2 μL DTT, 20 U ProtoScript ® II Reverse Transcriptase (New England Biolabs) and 125 μmol of each dNTP (12.5 μM) were combined in a 20 μL reaction mixture. .. Then, reverse transcription was performed in a FlexCycler PCR System (Analytik Jena, Germany) with the following protocol: 25 °C for 5 min, reverse transcription at 42 °C for 1 h followed by a step of 20 min at 65 °C for inactivation of the enzyme. .. The obtained cDNA was then used as the template (2 μL) in a 20 μL polymerase chain reaction (PCR) mix containing 500 nmol of the respective primer set to detect SBWMV, SBCMV or JSBWMV RNA1 or RNA2 , 125 μmol of each dNTP, 4 μL of 5× buffer and 0,5 U One Taq polymerase (New England Biolabs).



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Journal: iScience

Article Title: Real-time monitoring of deformed wing virus-infected bee foraging behavior following histone deacetylase inhibitor treatment

doi: 10.1016/j.isci.2021.103056

Figure Lengend Snippet:

Article Snippet: FlexCycler 2 PCR Thermal Cycler , VWR International, LLC. , 16055039.

Techniques: Recombinant, PCR Cloning, Plasmid Preparation, Western Blot, Software, Spectrophotometry, Real-time Polymerase Chain Reaction, Expressing